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- W2378597673 abstract "A new and simple method for bulk isolation and preparation of serum β-lipoprotein by pure chemical means was presented. By taking advantage of certain properties of the β-lipoprotein-dextran sulfate complex it was found possible to avoid ultracon- trifugation altogether.It was found that macromolecular dextran sulfate could interact specifically at neutral pH with serum β-lipoprotein to form an insoluble complex and that it did not react with serum α-lipoprotein or other serum proteins. The complex formed could be rendered soluble by increasing the ionic strength of the medium. The loosely combined macromolecular dextran sulfate was then removed by precipitating it with barium acetate, thus leaving the free β-lipoprotein in solution.The purity and properties of the isolated β-lipoprotein was characterized by means of paper electrophoresis, agar gel electrophoresis and immunoelectrophoretic and ultracentrifugal analyses. It was found that the isolated β-lipoprotein solution no longer containe an appreciable amount of dextran sulfate upon detecting the polyanions with acid polysaccharide dyes. On agar gel electrophoresis there appeared only one spot whose position corresponded to α_2-globulin. On paper electrophoresis there was revealed only one band corresponding to that of β-globulin of whole serum, whether it was stained with protein or lipid dyes. Upon ultracentrifugation with a maximum speed of 50,000 rpm it could float in a high density medium of 1.063g/ml. All the above data suggest that the isolated β-lipoprotein is quite pureUltracentrifugal analysis showed that the lipoprotein contained mainly the β-lipoprotein of S_f 2-6, but there was also a small amount of low density lipoprotein of higher S_f values. Immunoeletrophoretic analysis showed, however, only one precipitating arc with antiwhole serum, indicating that β-lipoproteins of different S_f values may have similar immunochemical properties.The newly prepared β-lipoprotein is transparent and light yellow in color and has 2 to 3sorption peaks in visible light, between 410mμ to 500mμ, and one in ultraviolet light at 275mμ. It can be stored at 2° to 4℃ for 14 days or concentrated at 4℃ without the formation of any precipitate.Finally, the characteristics of dextran sulfate-β-lipoprotein complex in concentrated salt solution was studied by stainning the protein and polyanions with Azo-carmine and Alcian Blue dyes on the same paper strip after electrophoresis. It was found that the complex was still in a state of combination and not at all dissociated." @default.
- W2378597673 created "2016-06-24" @default.
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- W2378597673 date "1964-01-01" @default.
- W2378597673 modified "2023-09-25" @default.
- W2378597673 title "STUDIES ON SERUM β-LIPOPROTEIN I. BULK ISOLATION AND PREPARATION OF HUMAN SERUM β-LIPOPROTEIN WITH MACROMOLECULAR DEXTRAN SULFATE AND ITS CHARACTERIZATION" @default.
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