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- W2378613563 abstract "Objective:To construct a recombinant vector of short hairpin RNA(shRNA) targeting TERF2IP1 gene,and to identify its inhibitory effect in HCT116 cells.Methods:the TERF2IP1 shRNA sequences were designed and inserted into pGPHI/Hygro vector.HCT116 cells were transiently transfect with shRNA vector and control vector,selected positive clone by hygromycin for 2~3 days.The total RNA and protein were extracted from the cells and the silence effect of TERF2IP1 was detected.Results:Four pair pGPHI/Hygro vector targeting TERF2IP1 were constructed,and the stably TERF2IP1 knockdown HCT116 cells were obtained.The expression of TERF2IP1 in HCT116 cells were downregulated significantly,confirmed by real-time PCR and Western blot.Conclusion:The constructed shRNA vector targeting human TERF2IP1 gene can stably suppress TERF2IP1 expression in HCT116 cells." @default.
- W2378613563 created "2016-06-24" @default.
- W2378613563 creator A5072140480 @default.
- W2378613563 date "2015-01-01" @default.
- W2378613563 modified "2023-09-23" @default.
- W2378613563 title "Construction of Recombinant HCT116 Cells with sh RNA Vector Targeting TERF2IP1 Gene" @default.
- W2378613563 hasPublicationYear "2015" @default.
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