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- W2378613787 abstract "Objective To study the antiviral effect of antigene oligodeoxynucleotides (ODNan) and antisense oligodeoxynucleotides (ODNas) on replication of hepatitis B virus(HBV). Methods A 21mer phosphorothioate ODNan (ODNan21) directed at Sp1 sites in hepatitis B virus(HBV) core promoter (1734 nt～1754 nt) and a 21mer phosphorothioate ODNas (ODNas21) complementary to the initiation site of pre C mRNA and pregenomic RNA (1813 nt～1833 nt) were synthesized. 22.1.5 cells were treated with these oligodeoxynucleotides. HBsAg, HBeAg, and HBV DNA were determined by enzyme linked immunosorbent assay(ELISA) and dot hybridization methods, respectively, in the treated 22.1.5 cells. Results The levels of HBsAg and HBeAg in ODNan21 group and ODNas21 group were lower than that in blank control group ( P 0.001). ODNan21 and ODNas21 of 10 μmol/L reduced synthesis of HBsAg, HBeAg by 57.5% and 61%, 77% and 79.6%, respectively, while the combination of ODNan21 and ODNas21 showed stronger inhibition to HBsAg and HBeAg (71.5% and 85%). The inhibitory effects were dose dependent, and reached to the highest point after 48 hours of administration. The HBV DNA was significantly decreased in the ODNan21 group as well as ODNas21 group. No inhibitory effects on the levels of HBsAg, HBeAg and HBV DNA were observed in the oligodeoxynucleotide control group. In virus inhibitory concentrations the oligodeoxynucleotides were harmless to 22.1.5 cells. Conclusion The results indicated that both antigene oligodeoxynucleotides and antisense oligodeoxynucleotides could partly inhibit the replication of HBV and synthesis of antigen." @default.
- W2378613787 created "2016-06-24" @default.
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- W2378613787 date "2000-01-01" @default.
- W2378613787 modified "2023-09-27" @default.
- W2378613787 title "Inhibition of hepatitis B virus replication and synthesis of antigen by antigene and antisense oligodeoxynucleotides in vitro" @default.
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