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- W2378622951 abstract "Aim To evaluate the effects of phenylalanine dipeptide compound Y101 on DHBV-DNA in vitro and vivo.Methods Beijing ducklings infected with duck hepatitis B virus were divided into control,Y101,Lamivudine groups.Serum was collected at 0,5 and 1 0 days after drugs were administered and at 3 days after drugs were withdrawn from vena cruralis.The presence of DHBV-DNA in serum was detected by dot blot hybridization;Duck primary hepatocytes were collected from Beijing ducklings infected with duck hepatitis B virus by perfusion.DHBV-DNA in cells and supernatants was extracted from Y101,Lamivudine and normal control groups with the use of QuickExtract DNA Extraction Soln 1.0.The contents of DHBV-DNA were determined with Taqman probe Quantitative RealTime PCR method and the inhibitory rates were calculated.Results Y101 could obviously inhibit DHBVDNA in vitro and vivo.The effective dosages were 0.05 g.kg-1,0.025 g.kg-1 groups in vivo and 80 mg.L-1,60 mg.L-1 groups in vitro,respectively.The content of DNA decreased significantly.Conclusion Y101 has a siginificant inhibiory effect on DHBV-DNA in vitro and vivo." @default.
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- W2378622951 date "2012-01-01" @default.
- W2378622951 modified "2023-09-25" @default.
- W2378622951 title "Effects of phenylalanine dipeptide compound Y101 on DHBV-DNA" @default.
- W2378622951 hasPublicationYear "2012" @default.
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