Matches in SemOpenAlex for { <https://semopenalex.org/work/W2378623273> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2378623273 abstract "Objective To explore the essential conditions of the normal tooth germ cells culture in order to use these cultured cells as the seeding cell in the tissue-engineering of tooth .Methods Take out the first mandibular molar tooth germs carefully from 17 days fetal SD rats in use of dissectal anatomic micrograph. Enamel and pulp organ tissues were enzymatically treated into single cell and cell units suspension and cultured in Low Glucose DMEM with 10% Fetal Cow Serum(FCS). Observe the cells growth characteristics on Covert Micrograph, and draw a growth curve after MTT essay. Identify these cells by morphology and immunohistochemistry . Results Cells adhered to the plates within 24 hours and growth in monolayer before confluence.Cells cultured in DMEM formed a mixed cell population of epithelial-like and fibroblast-like.The multilateral-shaped cells grow tightly in typical cobblestone appearance. The growth curve indicate that cells proliferate slowly in the first 3 days, then increased gradually. The two generation cells of tooth germ can be cultured in one medium by immunohistochemistry. Conclusions By enzymatically treated, collagen used as substrate, tooth germ cells could be mixed cultured and maintained its characteristics." @default.
- W2378623273 created "2016-06-24" @default.
- W2378623273 creator A5057614080 @default.
- W2378623273 date "2004-01-01" @default.
- W2378623273 modified "2023-09-24" @default.
- W2378623273 title "The Experimental Investigation of Fetal Rat’s Tooth Germ Cells Culture in Vitro" @default.
- W2378623273 hasPublicationYear "2004" @default.
- W2378623273 type Work @default.
- W2378623273 sameAs 2378623273 @default.
- W2378623273 citedByCount "0" @default.
- W2378623273 crossrefType "journal-article" @default.
- W2378623273 hasAuthorship W2378623273A5057614080 @default.
- W2378623273 hasConcept C105702510 @default.
- W2378623273 hasConcept C142724271 @default.
- W2378623273 hasConcept C1491633281 @default.
- W2378623273 hasConcept C16685009 @default.
- W2378623273 hasConcept C185592680 @default.
- W2378623273 hasConcept C202751555 @default.
- W2378623273 hasConcept C203014093 @default.
- W2378623273 hasConcept C204232928 @default.
- W2378623273 hasConcept C54355233 @default.
- W2378623273 hasConcept C55493867 @default.
- W2378623273 hasConcept C62112901 @default.
- W2378623273 hasConcept C71924100 @default.
- W2378623273 hasConcept C81885089 @default.
- W2378623273 hasConcept C86803240 @default.
- W2378623273 hasConcept C95444343 @default.
- W2378623273 hasConceptScore W2378623273C105702510 @default.
- W2378623273 hasConceptScore W2378623273C142724271 @default.
- W2378623273 hasConceptScore W2378623273C1491633281 @default.
- W2378623273 hasConceptScore W2378623273C16685009 @default.
- W2378623273 hasConceptScore W2378623273C185592680 @default.
- W2378623273 hasConceptScore W2378623273C202751555 @default.
- W2378623273 hasConceptScore W2378623273C203014093 @default.
- W2378623273 hasConceptScore W2378623273C204232928 @default.
- W2378623273 hasConceptScore W2378623273C54355233 @default.
- W2378623273 hasConceptScore W2378623273C55493867 @default.
- W2378623273 hasConceptScore W2378623273C62112901 @default.
- W2378623273 hasConceptScore W2378623273C71924100 @default.
- W2378623273 hasConceptScore W2378623273C81885089 @default.
- W2378623273 hasConceptScore W2378623273C86803240 @default.
- W2378623273 hasConceptScore W2378623273C95444343 @default.
- W2378623273 hasLocation W23786232731 @default.
- W2378623273 hasOpenAccess W2378623273 @default.
- W2378623273 hasPrimaryLocation W23786232731 @default.
- W2378623273 hasRelatedWork W1504851641 @default.
- W2378623273 hasRelatedWork W1993237873 @default.
- W2378623273 hasRelatedWork W2002347162 @default.
- W2378623273 hasRelatedWork W2066678011 @default.
- W2378623273 hasRelatedWork W2263950546 @default.
- W2378623273 hasRelatedWork W2353812857 @default.
- W2378623273 hasRelatedWork W2360061050 @default.
- W2378623273 hasRelatedWork W2394725084 @default.
- W2378623273 hasRelatedWork W2401176174 @default.
- W2378623273 hasRelatedWork W2414942853 @default.
- W2378623273 hasRelatedWork W2415536772 @default.
- W2378623273 hasRelatedWork W2462251429 @default.
- W2378623273 hasRelatedWork W2495840701 @default.
- W2378623273 hasRelatedWork W2512772495 @default.
- W2378623273 hasRelatedWork W2554257219 @default.
- W2378623273 hasRelatedWork W3003107535 @default.
- W2378623273 hasRelatedWork W3032712698 @default.
- W2378623273 hasRelatedWork W3147103796 @default.
- W2378623273 hasRelatedWork W2184160414 @default.
- W2378623273 hasRelatedWork W2602589095 @default.
- W2378623273 isParatext "false" @default.
- W2378623273 isRetracted "false" @default.
- W2378623273 magId "2378623273" @default.
- W2378623273 workType "article" @default.