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- W2378624938 abstract "To investigate the effect of cadmium on calcium homeostasis in cerebral cortical neurons of rat cultured in vitro,neurons specific enolase(NSE)antibody,recognized specifically NSE antigen in cultured cells,was used to identify cerebral cortical neurons of rat by immunocytochemical method.The neurons were exposured to different concentrations(0,5,10,20 μmol/L) of cadmium acetate for 12 hours.Then,intracellulari was detected by flow cytometry,the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were measured by ATPase test kits and the transcription level of CaM mRNA was detected by real-time fluorescent quantitative PCR.The neurons were affirmed by NSE staining which showed that cultured cells were positive staining by NSE antibody.In comparison with the control group,the results showed that intracellular i was increased significantly(P0.05 or P 0.01),the activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase were decreased significantly(P0.05 or P0.01),the transcription level of CaM mRNA was decreased significantly(P0.01) in 20 μmol/L group.It was suggested that cadmium could impair neurons by disturbing intracellular Ca2+ homeostasis and inhibiting the transcription level of CaM mRNA and activities of Na+-K+-ATPase and Ca2+-Mg2+-ATPase." @default.
- W2378624938 created "2016-06-24" @default.
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- W2378624938 date "2013-01-01" @default.
- W2378624938 modified "2023-09-27" @default.
- W2378624938 title "Effects of cadmium on calcium homeostasis in cerebral cortical neurons of rat cultured in vitro" @default.
- W2378624938 hasPublicationYear "2013" @default.
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