Matches in SemOpenAlex for { <https://semopenalex.org/work/W2378628935> ?p ?o ?g. }
Showing items 1 to 67 of
67
with 100 items per page.
- W2378628935 abstract "To investigate the role of P53 binding element in the regulation of NODS gene,the promoter region of this gene containing P53 consensus was amplified by PCR from human genomic DNA and correctly connected to the vector pEGFP-C2 which had cut out promoter by restriction enzyme to obtain the GFP expression vector driven by human NOD8 gene promoter.Mutagenesis of the constructed vector pEGFP-C2-NOD8wt to delete the P53 binding site was carried out by using the Quik hange site-directed mutagenesis kit.The constructed plasmids pEGFP-C2-NOD8wt and mpEGFP-C2-NOD8 were transiently transferred into cell line HEK293 by JetPeiTM and the GFP expression was observed under the condition of inversion fluorescence microscope.After the HEK293 were treated with PFT-α,an inhibitor of P53,at different concentrations for 24 h,the recombinant plasmid pEGFP-C2-NOD8 was transiently transferred into cell line HEK293 by JetPeiTM,and the GFP expression was observed.It was found the constructed pEGFP-C2-NOD8wt plasmids and mpEGFP-C2-NOD8 were the same as the design confirmed by restriction digestion and sequence analysis.The GFP expression of constructed pEGFP-C2-NOD8wt was stronger than that of mpEGFP-C2-NOD8wt(P0.01).PFT-α could inhibit the GFP expression of pEGFP-C2-NOD8wt in a concentration dependent manner(P0.05).In the end,the GFP expression vector driven by human NOD8 gene promoter containing P53 consensus site and the site of deleted plasmid were successfully constructed.The result of the cell transient transfection indicated that the GFP expression of recombinant plasmid mpEGFP-C2-NOD8,deleted the P53 binding site,was obviously weaken in HEK293 cells.In a dose-dependent manner,PFT-α down-regulated the expression of GFP in cells transfected with pEGFP-C2-NOD8wt plasmids via inhibiting the function of P53.These results indicate that P53 binding element may play a positive role in regulation of NOD8 gene,thus establishing a favourable base for further study on the mechanism of NOD8 gene regulation." @default.
- W2378628935 created "2016-06-24" @default.
- W2378628935 creator A5019081068 @default.
- W2378628935 date "2010-01-01" @default.
- W2378628935 modified "2023-09-25" @default.
- W2378628935 title "The role of P53 binding element in the regulation of NOD8 gene" @default.
- W2378628935 hasPublicationYear "2010" @default.
- W2378628935 type Work @default.
- W2378628935 sameAs 2378628935 @default.
- W2378628935 citedByCount "0" @default.
- W2378628935 crossrefType "journal-article" @default.
- W2378628935 hasAuthorship W2378628935A5019081068 @default.
- W2378628935 hasConcept C104317684 @default.
- W2378628935 hasConcept C128319531 @default.
- W2378628935 hasConcept C142613039 @default.
- W2378628935 hasConcept C150194340 @default.
- W2378628935 hasConcept C153911025 @default.
- W2378628935 hasConcept C16318435 @default.
- W2378628935 hasConcept C174510640 @default.
- W2378628935 hasConcept C22744801 @default.
- W2378628935 hasConcept C33161422 @default.
- W2378628935 hasConcept C40767141 @default.
- W2378628935 hasConcept C501734568 @default.
- W2378628935 hasConcept C54009773 @default.
- W2378628935 hasConcept C54355233 @default.
- W2378628935 hasConcept C86803240 @default.
- W2378628935 hasConceptScore W2378628935C104317684 @default.
- W2378628935 hasConceptScore W2378628935C128319531 @default.
- W2378628935 hasConceptScore W2378628935C142613039 @default.
- W2378628935 hasConceptScore W2378628935C150194340 @default.
- W2378628935 hasConceptScore W2378628935C153911025 @default.
- W2378628935 hasConceptScore W2378628935C16318435 @default.
- W2378628935 hasConceptScore W2378628935C174510640 @default.
- W2378628935 hasConceptScore W2378628935C22744801 @default.
- W2378628935 hasConceptScore W2378628935C33161422 @default.
- W2378628935 hasConceptScore W2378628935C40767141 @default.
- W2378628935 hasConceptScore W2378628935C501734568 @default.
- W2378628935 hasConceptScore W2378628935C54009773 @default.
- W2378628935 hasConceptScore W2378628935C54355233 @default.
- W2378628935 hasConceptScore W2378628935C86803240 @default.
- W2378628935 hasLocation W23786289351 @default.
- W2378628935 hasOpenAccess W2378628935 @default.
- W2378628935 hasPrimaryLocation W23786289351 @default.
- W2378628935 hasRelatedWork W1502563456 @default.
- W2378628935 hasRelatedWork W1567953207 @default.
- W2378628935 hasRelatedWork W1614962268 @default.
- W2378628935 hasRelatedWork W1927608037 @default.
- W2378628935 hasRelatedWork W1965988377 @default.
- W2378628935 hasRelatedWork W2001340411 @default.
- W2378628935 hasRelatedWork W2043847594 @default.
- W2378628935 hasRelatedWork W2046888994 @default.
- W2378628935 hasRelatedWork W2052266897 @default.
- W2378628935 hasRelatedWork W2084088697 @default.
- W2378628935 hasRelatedWork W2141190704 @default.
- W2378628935 hasRelatedWork W2141511486 @default.
- W2378628935 hasRelatedWork W2260311374 @default.
- W2378628935 hasRelatedWork W2358535682 @default.
- W2378628935 hasRelatedWork W2397675911 @default.
- W2378628935 hasRelatedWork W2401094510 @default.
- W2378628935 hasRelatedWork W3029192458 @default.
- W2378628935 hasRelatedWork W3031379481 @default.
- W2378628935 hasRelatedWork W3105411954 @default.
- W2378628935 hasRelatedWork W3176553922 @default.
- W2378628935 isParatext "false" @default.
- W2378628935 isRetracted "false" @default.
- W2378628935 magId "2378628935" @default.
- W2378628935 workType "article" @default.