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- W2378634813 abstract "Objective To prepare monoclonal antibodies against Neisseria meningococcal serogroup A polysaccharide(GAMP). Methods BALB/c mouse was immunized by GAMP-Tetanus Toxoid(TT).Hybridoma cell lines secreting monoclonal antibodies against GAMP were screened after the fusion of mouse splenic cells with SP2/0 cells.The number of chromosome in hybridoma cell was tested by colchicines-interception,and the ascite of mice was prepared by pristane-inducing method.The mAb was purified by octansic acid-ammonium sulfate precipitation,and purity of the extracted protein was measured by gray scale-scanning assay of SDS-PAGE.The specificity of anti-GAMP mAb was identified by neutration of antigen and antigen-substitute method. Results The hybridoma(2E7) had a healthy growth state,and the chromosome of which was 139.73.It produced proper amount of mAb,which was identified to be IgG1.The concentration of extracts was 1.76-2.32 mg·mL-1,and the purity was 97.2%.The potency of HRP-marked anti-GAMP mAb was 1:25 600,and the anti-GAMP E7 mAb showed a specificity for GAMP by antigen substitution and neutralization assay. Conclusion The anti-GAMP E7 mAb has been successfully obtained,and it makes a necessary foundation for the quality control and manufacture monitoring during preparation of the GAMP-TT vaccine." @default.
- W2378634813 created "2016-06-24" @default.
- W2378634813 creator A5038347628 @default.
- W2378634813 date "2011-01-01" @default.
- W2378634813 modified "2023-09-25" @default.
- W2378634813 title "Preparation of Monoclonal Antibody Against Neisseria Meningococcal Serogroup A Polysaccharide(GAMP) by Using the Conjugate Vaccine of both GAMP and Tetanus Toxoid" @default.
- W2378634813 hasPublicationYear "2011" @default.
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