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- W2378636020 abstract "Objective To detect the gene expression of eukaryotic recombinant plasmid pEGFP-FcγRIIb in eukaryotic cells and BALB/c mice,and to establish a foundation for the further study in vivo.Methods The NIH3T3 cells were transfected by the recombinant plasmid of pEGFP-FcγRIIb with lipofection strategy,using G418 to select positive cells for three weeks.The total protein was analyzed by Western blot to detect stable products.The BALB/c mice were injected intramuscularly with the pEGFP-FcγRIIb,then the expression of human FcγRIIB was detected with Western blot and Immunohistochemistry.Results About 37 kDa protein of human FcγRIIB was detected in stable transfected NIH3T3 cells and in BALB/c mice which were injected the pEGFP-FcγRIIb.Conclusion The success in expression of the eukaryotic recombinant plasmid pEGFP-FcγRIIb in the NIH3T3 cells and BALB/c mice make it possible to study further." @default.
- W2378636020 created "2016-06-24" @default.
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- W2378636020 date "2012-01-01" @default.
- W2378636020 modified "2023-09-25" @default.
- W2378636020 title "Expression of Eukaryotic Recombinant Plasmid pEGFP-FcγRIIb in Eukaryotic Cells and BALB/c Mice" @default.
- W2378636020 hasPublicationYear "2012" @default.
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