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- W2378639214 abstract "Objective:To establish a method for determining pioglitazone hydrochloride in human plasma.Methods:A high performance liquid chromatographic assay was developed. The plasma sample was extracted with diethyl ether. The sample was separated on HiQsil C18(4.6 mm×150 mm,5 μm),with KH 2PO 4(pH=6.30):CH 3OH:CH 3CN(3:2:3.5)as the mobile phase (flow rate at 1.0 ml/min ).The determining wavelength was 230 nm. Results:The calibration curve was linear over the range of 25 ng/ml～3200 ng/ml(γ= 0.9994, n =12),the lowest limit of detection was 25 ng/ml.The absolute recoveries of high,medium and low concentrations were:70.63%±2.61%,76.05%±4.31% and 82.10%±6.92%,respectively. The relative recoveries of high, medium and low concentration were 101.24%±6.74%,106.16%±7.89% and 90.77%±2.87%,respectively.Within day RSD7.41%( n =5)and between day RSD5.24%( n =5). Conclusion:This study provided a reliable quantitative method for pharmacokinetic study of pioglitazone hydrochloride." @default.
- W2378639214 created "2016-06-24" @default.
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- W2378639214 date "2002-01-01" @default.
- W2378639214 modified "2023-09-23" @default.
- W2378639214 title "High-performance liquid chromatographic determination of pioglitazone hydrochloride in human plasma" @default.
- W2378639214 hasPublicationYear "2002" @default.
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