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- W2378640465 abstract "Objective:To construct plasmid with overexpressed human SGK1 to provide a useful tool for its studies in vitro.Methods:the full length SGK,was amplified with cDNA,cDNA reverse transcriped from cell total-RNA,then constructed into the expression vector.The active and dominant-negative human SGK1 were obtained by site-directed mutagenesis.All SGK1 plasmids were verified by digestion with restriction enzymes and DNA sequencing.The efficiency of transfection of recombinant SGK1 was analysed in HEK293 cells by phosphate-calcium deposit method.Results:Recombinant human SGK1 is successfully over-expressed in the HEK293 cells.Conclusion:The successful construction of over expressed human SGK1 developed a new technique for studying the pathogenesis of diabetes nephrology." @default.
- W2378640465 created "2016-06-24" @default.
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- W2378640465 date "2006-01-01" @default.
- W2378640465 modified "2023-09-28" @default.
- W2378640465 title "Construction and Identification of Over Expressed Human SGK1" @default.
- W2378640465 hasPublicationYear "2006" @default.
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