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- W2378640622 abstract "Electrotransformation, a highly efficient and easy-applying technique, is used to introduce vector pTEF1 / Zeo-rDNA into Rhodotorula benthica in this work. The optimal electroporated stage for Rhodotorula benthica was at intermediate logarithmic phage. Under the conditions of voltage of 900 V and 20 mg / L plasmid DNA and 0.2 cm cuvettes, the transformation efficiency reach its maximum at 52 transformants / μg plasmid DNA. The results showed that all of the transformants were positive clones. The experiments provided a model for highly efficient electrotransformation of Rhodotorula benthica as host cells for the first time. The research established a basis of studying biological properties of Rhodotorula benthica and its further development of genetic engineering products." @default.
- W2378640622 created "2016-06-24" @default.
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- W2378640622 date "2008-01-01" @default.
- W2378640622 modified "2023-09-26" @default.
- W2378640622 title "The Study of Conditions of Electrotransformation in Rhodotorula benthica" @default.
- W2378640622 hasPublicationYear "2008" @default.
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