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- W2378645480 abstract "In order to obtain optimal extraction method of high quality total RNA from Vaccinium dunalianum leaf buds,improved CTAB,improved SDS,RNApure Plant Kit and Plant RNA Kit methods were used comparatively.The quality of total RNA was examined through OD value and agarose gel electrophoresis.The results showed that using improved CTAB and Plant RNA Kit methods can obtain high purity and quality RNA.The ratios of A260/A280 were 2.07 and 2.08 respectively,the brightness of 28S rRNA was twice that of 18S rRNA,and the total RNA obtained was intact and pure.A partial cDNA with 316 bp length of anthocyanin 5-aromatic acyltransferase gene from V.dunalianum was cloned by RT-PCR with RNA as templates obtained by improved CTAB and Plant RNA Kit methods.The results showed that improved CTAB and Plant RNA Kit methods were the better methods to extract high quality total RNA from V.dunalianum leaf buds." @default.
- W2378645480 created "2016-06-24" @default.
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- W2378645480 date "2013-01-01" @default.
- W2378645480 modified "2023-09-24" @default.
- W2378645480 title "The Comparison on Total RNA Extraction Methods from Vaccinium dunalianum Leaf Buds" @default.
- W2378645480 hasPublicationYear "2013" @default.
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