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- W2378664491 abstract "Objective To study the effect of endotoxin on the hepatic apoptosis and the role of endotoxin in severe hepatitis.Method To stimulate the L02 hepatocyte 4h,8h,16h,24h,respectively with endotoxin 10mg/ml,to detect the hepatic apoptosis by flow cytometry and fluorescein stain,and to measure the expression of smac and caspase9 by immunohistochemistry as well.Result L02 cell morphologically displayed cytoplasm concentrated,nuclear-mass rally,the formation of apoptotic bodies after the stimulation of LPS;the LPS-stimulated L02 cells were stained with Hoechst and were observed under fluorescence microscope,which emit blue fluorescence.The late apoptotic rates were 2.3%,4.0%,6.2%,18.2%,respectively with flow analysis after 4h,8h,16h,24h with LPS stimulation.The apoptotic rates increased with time and there were significant differences(P0.05) compared with the normal control group which apoptotic rate was 0.3%,the expression of protein smac and caspase9 increased with the increasing of stimulation time(P0.01).Compared with the normal control group,the expression of protein smac and caspase9 detected with immunohistochemistry significantly increased in all of the stimulation groups(P0.05).Caspase9 and smac protein diplayed brown in the cytoplasm,smac protein was positive correlated with the expression of Caspase9(r=0.527,P0.001).Conclution LPS in vitro can induce apoptosis of normal liver cell by pro-apoptotic protein smac and can enhance the activity of Caspase9 of the downstream pathway." @default.
- W2378664491 created "2016-06-24" @default.
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- W2378664491 date "2008-01-01" @default.
- W2378664491 modified "2023-09-24" @default.
- W2378664491 title "The role of pro-apoptotic proteins smac and caspase9 in lipopolysaccharide-induced apoptosis of hepatocyte in vitro" @default.
- W2378664491 hasPublicationYear "2008" @default.
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