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- W2378694334 abstract "Objective To evaluate the feasibility of colloidal gold assay on syphilis diagnosis,and to analyze the influence factors of syphilis weakly positive.Methods Serum specimen of 342 syphilis and 362 no-syphilis diagnosed by TPPA were tested by TRUST,ELISA and colloidal gold assay.Colloidal gold assay sensitivity was analyzed by ROC curve and the multi-proportion dilute of syphilis Serum specimen.The effects of pH, haemolysis and lipidemia on colloidal gold assay testing for syphilis were analyzed by testing different pH, haemolysis and lipidemia syphilis Serum specimen.Results The missed diagnosis rate of colloidal gold assay was 9.30%and false positive rate was 27.07%.Area under the curve was 0.817 and standard error was 0.017.Testing result of colloidal gold assay was stable and reliable when pH value was between 6.0 and 8. 0.Testing result was failed completely when pH value was lower than 2.0 or higher than 11.0.Haemolysis and lipidemia of specimen had no effect on treponema pallidum antibody when the concentration was high, but under the condition of low concentration,it proved to be false negative.Conclusion When treponema pallidum antibody concentration is low,the sensitivity of colloidal gold diagnostic is poor,meanwhile haemolysis and lipidemia have interference in the detection,so it isn' t suitable for Outpatient services screening" @default.
- W2378694334 created "2016-06-24" @default.
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- W2378694334 date "2012-01-01" @default.
- W2378694334 modified "2023-09-23" @default.
- W2378694334 title "Value of Colloidal Gold Assay on Syphilis Diagnosis and Influence Factors of Its Weakly Positive" @default.
- W2378694334 hasPublicationYear "2012" @default.
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