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- W2378697195 abstract "In the previous study, the monoclonal antibodies were utilized, which was corresponding to HS2-binding proteins, to screen λ-gtll human cDNA expression library of fetal liver. In this way, two cDNA clones named EDRF1 and EDRF2 were obtained respectively. This study focuses on the function of erythroid related factor 1, which was identified that it owns a whole sequence, by transfecting K562 cells characterized by stable expression from the evidence of genomic integration of Neo gene by G418 selection. EDRF1 mRNA expression was suppressed in pcDNA3 antisense construct plasmid trasfected K562 cells identified by Northern blot. It suggests that antisense RNA expression disturbed that synthesis of EDRF1 mrna efficiently. Both γ-globin and PKC-α mRNA expressions were down-regulated in antisense construct plasmid transfected K562 cells demonstrated by semiquantitative RT-PCR. It suggests that the inhibition of PKC signal may be the negative modulation of K562 differentiation and globin synthesis." @default.
- W2378697195 created "2016-06-24" @default.
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- W2378697195 date "2001-01-01" @default.
- W2378697195 modified "2023-09-24" @default.
- W2378697195 title "Antisense RNA of a novel erythroid related gene EDRF1 down-regulates" @default.
- W2378697195 hasPublicationYear "2001" @default.
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