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- W2378699543 abstract "Autocatalytic protein splicing mediated by intein is carried out with 100% efficiency in primitive species. However, the splicing activity will decrease greatly or even be lost completely when the protein was expressed in heterologous hosts. In our study, several short extein coding sequences were designed and introduced at different insertion sites into a kanamycin resistance gene (kan~R) by PCR, according to the relationship between the splicing activity and the first amino acids of C-terminal extein. A whole set of kanamycin resistance-based screening system of intein was established in E.coli by inserting corresponding intein into these exteins. The results showed on the plate containing kanamycin high similarity to Western blot results, which indicated that the screening system of active intein was established successfully. By combining the splicing activity of intein with kanamycin resistance, we can use this system that contain all the facultative exteins to screen active intein only on the plates containing kanamycin without doing Western blotting any more, which can be used as a rapid, flexible, new approach to screen inteins that can splice in heterologous hosts." @default.
- W2378699543 created "2016-06-24" @default.
- W2378699543 creator A5018544698 @default.
- W2378699543 date "2009-01-01" @default.
- W2378699543 modified "2023-09-26" @default.
- W2378699543 title "An Efficient Screening System of Active Intein in Heterologous Hosts" @default.
- W2378699543 hasPublicationYear "2009" @default.
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