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- W2378703828 abstract "Objective To develop a new quantitative method for determining solasodine in Solanum nigrum L.by high-performance liquid chromatography(HPLC).Methods After sonic extracted with acidic methanol,the alkaloids were acid hydrolyzed with reflux,and then eluted with acetonitrile-0.05% heptafluorobutyric acid as mobile phase.The chromatography parameters were as follows: column: Diamonsil ODS analytical column;flow rate:1.0 ml/min;detection wavelength: 210 nm.Results Baseline separation was obtained for the alkaloids.Good linear range was found in the concentration range of 102~612 μg/ml with r= 0.999 9.The recoveries were no less than 96.0%,RSD was 3.35%(n=6).Conclusion The proposed method is simple,fast,accurate and effective.It can be used as a powerful tool for quality control of Solanum nigrum L.,also prvides a scientific and technical platform for setting up a quality standard and for further pharmaceutical study." @default.
- W2378703828 created "2016-06-24" @default.
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- W2378703828 date "2009-01-01" @default.
- W2378703828 modified "2023-09-26" @default.
- W2378703828 title "Determination of Solasodine in Solanum nigrum L. by High-performance Liquid Chromatography" @default.
- W2378703828 hasPublicationYear "2009" @default.
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