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- W2378713085 abstract "Objective To determine the 3′-terminal sequence of P RNA transcript and its form in Giardia.Methods Total RNA of the trophozoites of Giardia were extracted and polyadenylated,then reversely transcribed for 3′RACE with specific and adapter primers.The obtained cDNA with a poly A tail was identified by PCR and sequencing,of which the 3′-terminus was determined.The total expression levels of co-transcripted RNase P-GLsR15 RNA and matured RNase P RNA were determined by real-time fluorescent quantitative PCR(Rt-qPCR).Results The length of RNase P RNA 3′ cDNA was about 300 nt,of which the sequence at 3′-terminus was consistent with that of GLsR15.No significant differences were observed in total expression levels of co-transcripted RNase P-GLsR15 RNA and matured RNase P RNA(P 0.05).Conclusion The 3′-terminal sequence of RNase P RNA was successfully cloned,which confirmed that the cotranscripted RNase P-GLsR15 RNA was the existing form of matured RNase P RNA." @default.
- W2378713085 created "2016-06-24" @default.
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- W2378713085 date "2013-01-01" @default.
- W2378713085 modified "2023-09-24" @default.
- W2378713085 title "The 3′-terminal sequence of P RNA transcript and its form in Giardia" @default.
- W2378713085 hasPublicationYear "2013" @default.
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