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- W2378714302 abstract "Objective To analyze the secondary structure and B,T cell epitopes of the oyster major allergen Cra g 1,develop the immunotherapy of oyster allergy based on antigenic epitopes.Methods The amino acid sequence of oyster major allergen Cra g 1 was acquired from Uniprot database.The secondary structure of Cra g 1 was analyzed by DNAStar-Protean software with Garnier-Robson method and Chou-Fasman method.The B cell epitopes of Cra g 1 were analyzed synthetically by Kyte-Doolittle method,Karplus-Schulz method,Emini method and Jameson-Wolf method.The T cell epitopes of Cra g 1 were analyzed by SYFPEITHI and NetMHC-Ⅱ online website.Results The analysis of secondary structure showed Cra g 1 was mainly constituted by α-spiral.B cell epitopes covered almost the whole amino acid sequence of Cra g 1.However,T cell epitopes analysis demonstrated that the regions of 89-103,108-122 and 129-143 were the dominant T cell epitopes.Conclusion This study,which analyzed the dominant B,T cell epitopes of oyster allergen Cra g 1,provides a theoretical basis for further basic immunology researches of Cra g 1,such as vaccine development,diagnosis reagents preparation,and its antigenicity modification." @default.
- W2378714302 created "2016-06-24" @default.
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- W2378714302 date "2013-01-01" @default.
- W2378714302 modified "2023-09-24" @default.
- W2378714302 title "Analysis of the secondary structure, B cell and T cell epitopes of oyster allergen Cra g 1" @default.
- W2378714302 hasPublicationYear "2013" @default.
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