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- W2378715424 abstract "Enzymatic membrane reactor(EMR) with immobilization of polyphenol oxidase(PPO) is paid more attention due to its isolation and biochemical characteristics.In this paper,a enzymatic membrane immobilized PPO was prepared based on the chelation of PPO and Cu2+ in chitosan(CS)-Cu complex(CCC) with CS membrane as the carrier.The response surface methodology was applied to investigate the influence of three factors of copper ion sequestration,QCu2+,PPO concentration,CE,pH in immobilization process on the vitality of PPO immobilized in CCC.The relative activity of PPO in the solution and immobilized in the membrane was determined respectively.The characteristics of CS-Cu PPO membrane were discussed primarily.The results show that the optimal conditions for immobilization of PPO into the CCC were temperature 40 ℃,pH 7.67,QCu2+ 30.07 mg/mL,CE 2.12 mg/mL.Michaelis constant and the relative activity of PPO immobilized in CCC were larger than that of the solution.Enzyme half-decline period were 30 days,and the relative activity of PPO after repeated use of eight times reduced to 79% for CS-Cu-PPO membrane.This work offers the significantly basis for development of EMR with special function." @default.
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- W2378715424 date "2012-01-01" @default.
- W2378715424 modified "2023-09-25" @default.
- W2378715424 title "Characteristics and Immobilization Process of CS-Cu Enzymatic Membrane" @default.
- W2378715424 hasPublicationYear "2012" @default.
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