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- W2378723887 abstract "Objective To investigate the different cell-killing and bystander effects on GBC-SD cells with double suicide genes (cytosine deaminase and HSV-tk) transferred by retroviral vector and to find a more efficient and low-toxicity suicide gene therapy for gallbladder cancer. Methods Cytosine deaminase (CD) and HSV-tk double suicide genes were transfected into PA317 cells using lipofectamine. The positive clones were picked out and cultured after G418 was selected. The viral supernatant was collected and the GBC-SD cells were infected with the virus containing the double suicide genes. After G418 selection, the GBC-SD/CD+tk cells were collected and treated with 5-FC and/or GCV. The cytotoxicity efficacy was evaluated by MTT. Furthermore, the GBC-SD/CD+tk and GBC-SD cells were treated by 5-FC and GCV at different mixing ratios to explore their bystander effects. Results The virus containing double suicide gene was produced in PA317 cells and the cell line GBC-SD/CD+tk that expressed double suicide genes was acquired. The killing effect of 5-FC or GCV on GBS-SD/CD+tk cells was effective. The killing effect was more effective by using 5-FC in combination with GCV than by using 5-FC or GCV alone. When the ratio of GBC-SD/CD+tk cells reached 20%, 50% of the cells were killed. Conclusions The cell-killing efficacy of double suicide genes is higher than that of single suicide gene. Meanwhile, its bystander effect is obvious." @default.
- W2378723887 created "2016-06-24" @default.
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- W2378723887 date "2005-01-01" @default.
- W2378723887 modified "2023-09-27" @default.
- W2378723887 title "Killing effects of cytosine deaminase gene in combination with HSV-tk gene on gallbladder cancer cells" @default.
- W2378723887 hasPublicationYear "2005" @default.
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