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- W2378734227 abstract "Aureobasidium pullulans AP8,an original strain conserved in our laboratory,was treated with both EMS(ethyl methyl sulfonate) and UV(ultraviolet) to screen a high yield strain for pullulan production.The best mutation results were obtained through EMS treatment for 40 —60 min at a final concentration of 0.4 mol/L followed by UV irradiation for 1.5—2.5 min at a distance of 30 cm.A genetically stable mutant named as UV60 was obtained which is high in pullulan production(22.1 g/L) and low in pigment content.The mutant strain was markedly superior to the starting strain AP8 in terms of colony color,size and texture as well as fermentation performance.Using one-factor-at-a-time and orthogonal array design methods,the optimal shake fermentation medium for this mutant to produce pullulan was composed of 50.0 g/L sugar,1.5 g/L yeast extract,1.5 g/L NaCl,0.3 g/L MgSO4,2.0 g/L K2HPO4and 0.7 g/L(NH4)2SO4,resulting in a pullulan yield of 27.24 g/L and a conversion rate of 54.48% for polysaccharides." @default.
- W2378734227 created "2016-06-24" @default.
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- W2378734227 date "2012-01-01" @default.
- W2378734227 modified "2023-09-25" @default.
- W2378734227 title "Combined Mutation Screening of High Yield Strain for Pullulan Production" @default.
- W2378734227 hasPublicationYear "2012" @default.
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