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- W2378745596 abstract "Objective:To observe the influence of BHO on the promotion of rabbit fibroblasts proliferation.Methods:Fibroblasts of rabbit were obtained from the digested and aparted method of primary culture and identified by morphology and immunohistochemistry.The third purified fibroblasts were divided into 5 groups.Each hole of the blank compared group was added into same quantity PBS to culture.Each hole of the experimental groups was cultured respectively with the concentration of BHO of different quantity(125,62.5,31.25,15.625 mg/ml).MTT method was used to measure OD number of each hold after being cultured for 24,48, 72 h respectively.Results:Fibroblasts of rabbit were obtained from technology of cell culture and identified by morphology and immunohistochemistry.Compared with blank groups,OD numbers with different concentration BHO were obviously different(P0.05).Conclusion:Fibroblasts of rabbit could be obtained from the digested and aparted method of primary cell cluture;②BHO of regular concentration could increase the numbers of fibroblast obviously." @default.
- W2378745596 created "2016-06-24" @default.
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- W2378745596 date "2006-01-01" @default.
- W2378745596 modified "2023-09-23" @default.
- W2378745596 title "Research of immunohistochemistry on the influence of borneol and honey ointment(BHO)promote proliferation of the fibroblasts of rabbits" @default.
- W2378745596 hasPublicationYear "2006" @default.
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