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- W2378755655 abstract "Objective To compare the results of agarose gel electrophoresis and single stranded configuration pleomorphism(SSCP)analysis in detecting T cell receptor(TCR)γ gene rearrangement,explore the effective methods for TCRγ gene rearrangement detection.Method DNA extracted from T,B cell lymphomas and reactive lymphoid tissues were respectively submitted to two groups of TCRγgene rearrangement universal primers for PCR amplification,and PCR products were separately proceeded to agarose gel electrophoresis and SSCP.Results Clonal TCRγgene rearrangement presented both in T and B cell lymphomas.In SSCP of the two groups of PCR products,positive ratios of T cell lymphoma were 77.6% and 75.9%,respectively,while in B cell lymphomas,the positive ratios were both 10%.Compared to SSCP,false positive ratios in agarose gel electrophoresis were 5.3% and 14.4%,respectively.All the reactive lymphoid tissue emerged false positive.Conclusions Both T and B cell lymphomas exist clonal TCRγ gene rearrangement.Detection with agarose gel electrophoresis alone may result false positive,and the sensitivity of SSCP is higher." @default.
- W2378755655 created "2016-06-24" @default.
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- W2378755655 date "2009-01-01" @default.
- W2378755655 modified "2023-09-23" @default.
- W2378755655 title "Application of agarose gel electrophoresis and SSCP in lymphoma clonal TCRγ gene rearrangement detecting" @default.
- W2378755655 hasPublicationYear "2009" @default.
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