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- W2378775990 abstract "To obtain multidrug-resistance regulatory protein MarA of Shigella flexneri,the marA gene was amplified by PCR and subcloned into the vector pET-30a(+).The results of digestion with BamHⅠ and SalⅠ,PCR identification and sequencing analysis showed that the recombinant plasmid was successfully constructed.Then the recombinant plasmid was transformed into E.coli L21(DE3) competent cells for expression.The expressed protein was purified with Ni-NTA Column under denaturing conditions.The expressed MarA accounted for 30.2% of the total bacterial proteins.Western-blotting revealed that the expressed protein could be recognized specifically by antiserum against S.flexneri,which suggested the expressed protein MarA had a good immunoreactivity." @default.
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- W2378775990 date "2008-01-01" @default.
- W2378775990 modified "2023-09-25" @default.
- W2378775990 title "Prokaryotic expression of multidrug-resistance regulatory protein MarA of Shigella flexneri" @default.
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