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- W2378783224 abstract "Objective To study the differential gene expression of several collagens between human tenocytes and dermal fibroblasts in different individuals. Methods The samples of tendon and skin were obtained from discarded tissues of traumatic patients. The tissues were digested by enzyme to obtain tenocytes and dermal fibroblasts and cultured in vitro to passage 2. Then the comparison of collagen mRNA expression was made between the two types of cells using gene chip and RT-PCR. Results The morphology of two types of cells were similar to each other when observed under phase contrast microscope. RT-PCR, and gene chip showed that most collagens investigated in human tenocytes and dermal fibroblasts were similarly expressed even though two cell types were derived from the different individuals. Conclusion The passage 2 cells of human tenocytes and dermal fibroblasts cultured in vitro have similar collagen expression in different individuals. Human dermal fibroblasts may potentially be used as seed cells for tendon tissue engineering." @default.
- W2378783224 created "2016-06-24" @default.
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- W2378783224 date "2006-01-01" @default.
- W2378783224 modified "2023-09-25" @default.
- W2378783224 title "Comparative Study of Collagen Expression between Human Tenocytes and Dermal Fibroblasts in Different Individuals" @default.
- W2378783224 hasPublicationYear "2006" @default.
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