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- W2378789553 abstract "Objective: To construct lentivirus vector expressing mouse ameloblastin gene,it will provide a foundation for studying the function of ameloblastin in further study. Methods: RT-PCR was performed to get mouse ameloblastin coding region cDNA from spe-cial total RNA which was obtained from tooth germ at the bell stage of postnatal 1day, and coding region cDNA was cloned into pCRI-I-TOPO, then lentivirus vector pNL-IRES2-EGFP containing ameloblastin was constructed. The positive recombinant pNL-amelo-blastin-IRES2-EGFP were transfected into 293T cells, and collected lentivirus, moreover, identified their ability of infection. Results: pNL-ameloblastin-IRES2-EGFP was Constructed successfully, which was detected by restriction enzyme digestion analysis and PCR. Lentivirus produced in 293T infected human dental pulp stem cells(DPSCs), then fluorescence protein and ameloblastin can be ex-pressed stably in DPSCs. Conclusions: We have constructed recombinant lentivirus vector expressing mouse ameloblastin gene(pNL-ameloblastin-IRES2-EGFP),and produced lentivirus with ability of infection." @default.
- W2378789553 created "2016-06-24" @default.
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- W2378789553 date "2008-01-01" @default.
- W2378789553 modified "2023-09-24" @default.
- W2378789553 title "Construction and Expression of Recombinant Lentivirus Vector Expressing Mouse Ameloblastin Gene" @default.
- W2378789553 hasPublicationYear "2008" @default.
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