Matches in SemOpenAlex for { <https://semopenalex.org/work/W2378836406> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2378836406 abstract "To further study the proliferation and multi-differentiation potentials of adipose-derived stem cells(ADSCs),the cells were isolated with the improved methods and their growth curves were achieved with cck-8.Surface protein expression was analyzed by flow cytometry to characterize the cell phenotype by PT-PCR.The multi-lineage potential of ADSCs was testified by differentiating cells with adipogenic,chondrogenic,osteogenic and myogenic inducers.The results show that about 5× 105 stem cells can be obtained from 400-600mg adipose tissue.And ADSCs can be continuously cultured in vitro for up to 1month without passage and they have several logarithmic growth phases during the culture period.The relative surface marker expressions of all proliferated cells are positive.Meanwhile,stem cell related transcription factors,Nanog,Oct-4,Sox-2and Rex-1,are strongly positively expressed in ADSCs.After induced differentiation toward adipogenic,osteogenic,chondrogenic and myogenic cells,the differentiated ADSCs can respectively express intracellular lipid,alkaline and calcified nodules,mucopolysaccharide-rich extracellular matrix,and a little Connexin-43,which illustrates the ability of multi-lineage differentiation of ADSCs.In order to obtain more stem cells,ADSCs are subcultured every 14dinstead of normal 5daccording to growth curve.ADSCs still keep strong proliferation ability,maintain their phenotypes,and have stronger multi-differentiation potential." @default.
- W2378836406 created "2016-06-24" @default.
- W2378836406 creator A5024016708 @default.
- W2378836406 date "2010-01-01" @default.
- W2378836406 modified "2023-09-25" @default.
- W2378836406 title "Study of biological characteristics of adipose tissue-derived stem cell" @default.
- W2378836406 hasPublicationYear "2010" @default.
- W2378836406 type Work @default.
- W2378836406 sameAs 2378836406 @default.
- W2378836406 citedByCount "0" @default.
- W2378836406 crossrefType "journal-article" @default.
- W2378836406 hasAuthorship W2378836406A5024016708 @default.
- W2378836406 hasConcept C104317684 @default.
- W2378836406 hasConcept C107459253 @default.
- W2378836406 hasConcept C122927707 @default.
- W2378836406 hasConcept C145103041 @default.
- W2378836406 hasConcept C148738053 @default.
- W2378836406 hasConcept C171089720 @default.
- W2378836406 hasConcept C185592680 @default.
- W2378836406 hasConcept C189165786 @default.
- W2378836406 hasConcept C198826908 @default.
- W2378836406 hasConcept C207701140 @default.
- W2378836406 hasConcept C28328180 @default.
- W2378836406 hasConcept C35496256 @default.
- W2378836406 hasConcept C55493867 @default.
- W2378836406 hasConcept C86803240 @default.
- W2378836406 hasConcept C95444343 @default.
- W2378836406 hasConceptScore W2378836406C104317684 @default.
- W2378836406 hasConceptScore W2378836406C107459253 @default.
- W2378836406 hasConceptScore W2378836406C122927707 @default.
- W2378836406 hasConceptScore W2378836406C145103041 @default.
- W2378836406 hasConceptScore W2378836406C148738053 @default.
- W2378836406 hasConceptScore W2378836406C171089720 @default.
- W2378836406 hasConceptScore W2378836406C185592680 @default.
- W2378836406 hasConceptScore W2378836406C189165786 @default.
- W2378836406 hasConceptScore W2378836406C198826908 @default.
- W2378836406 hasConceptScore W2378836406C207701140 @default.
- W2378836406 hasConceptScore W2378836406C28328180 @default.
- W2378836406 hasConceptScore W2378836406C35496256 @default.
- W2378836406 hasConceptScore W2378836406C55493867 @default.
- W2378836406 hasConceptScore W2378836406C86803240 @default.
- W2378836406 hasConceptScore W2378836406C95444343 @default.
- W2378836406 hasLocation W23788364061 @default.
- W2378836406 hasOpenAccess W2378836406 @default.
- W2378836406 hasPrimaryLocation W23788364061 @default.
- W2378836406 hasRelatedWork W1991880649 @default.
- W2378836406 hasRelatedWork W2015028870 @default.
- W2378836406 hasRelatedWork W2056871333 @default.
- W2378836406 hasRelatedWork W2060672521 @default.
- W2378836406 hasRelatedWork W2067872386 @default.
- W2378836406 hasRelatedWork W2070728964 @default.
- W2378836406 hasRelatedWork W2120267080 @default.
- W2378836406 hasRelatedWork W2371659411 @default.
- W2378836406 hasRelatedWork W2374648502 @default.
- W2378836406 hasRelatedWork W2377843629 @default.
- W2378836406 hasRelatedWork W2463747892 @default.
- W2378836406 hasRelatedWork W2903077010 @default.
- W2378836406 hasRelatedWork W2903588164 @default.
- W2378836406 hasRelatedWork W2981526134 @default.
- W2378836406 hasRelatedWork W2981640286 @default.
- W2378836406 hasRelatedWork W2992015888 @default.
- W2378836406 hasRelatedWork W3031048975 @default.
- W2378836406 hasRelatedWork W3031346943 @default.
- W2378836406 hasRelatedWork W3205415805 @default.
- W2378836406 hasRelatedWork W2188385413 @default.
- W2378836406 isParatext "false" @default.
- W2378836406 isRetracted "false" @default.
- W2378836406 magId "2378836406" @default.
- W2378836406 workType "article" @default.