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- W2378855262 abstract "The paper collected oocytes from the sheep ovary by using 3 methods: the syrine aspiration,slicing and syrine aspiration combining with slicing,and then compared the oocytes number and time consuming to acquire A, B and C standard sheep oocytes. The results showed that the time consuming are 136.07 s and 131.60 s by syrine aspiration combining with slicing and slicing method,respectively.both of them are significantly higher than the time consuming by syrine aspiration,which time is 61.33 s(P0.05); slicing is the best way to collect 8 available oocytes(including A and B sheep oocytes).compared the 3 kinds medium with different hormone dose, all the maturation rate is higher above 81%,however, the difference is not significant, the maturation medium: TCM199- HCO3+ 10%FBS+l μg· mL-1E2+2.2 g· mL-1NaHCO3+50 μg· mL-1Gentamycin+5 μg· mL-1FSH+0.38 mmol· L-1NaPyruvate 1 IU· mL-1LH +10 mmol· L-1Hepes can be obtained the highest oocytes maturation rate 83.4%;83.4%; the sheep oocytes conserve in 10- 15 ℃, 15-20 ℃ and 20-30 ℃, all the oocytes maturation rate is 82% above, the difference is not significant; IA23187 and 6-DMAP be used for parthenogenetic activation, parthenogenetic activation embryo rate is the highest 91.5%." @default.
- W2378855262 created "2016-06-24" @default.
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- W2378855262 date "2014-01-01" @default.
- W2378855262 modified "2023-09-26" @default.
- W2378855262 title "Comparsion of different collecting oocytes method and in vitro maturation on sheep" @default.
- W2378855262 hasPublicationYear "2014" @default.
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