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- W2378889234 abstract "Objective To establish a HPLC method for simultaneous determination of puerarin, daidzin, and genistein from Pueraria lobata(wild) Ohwi, Pueraria thomsonii Benth and Pueraria mirifica. Methods The Hypersil BDS C18 column(250 mm×4.6 mm, 5 μm) was adopted. The mixture of methanol and water was used as mobile phase of gradient elution, with the flow rate of 0.7 m L/min. The injection volume was 10 μL. The Column temperature was 25 ℃, and the detection wavelength was 250 nm. Results This method had good linearity in the range of 0.05-1.20 μg, 0.025-0.60 μg and 0.025-0.60 μg for puerarin, daidzin and genistein, respectively, with r of 0.9999, 0.9999 and 0.9999, respectively. The average recoveries were 97.26 %-104.64 %, and RSDs were less than 3 %. Conclusion The method is simple, fast, reproducible, and with accurate and reliable results, which can provide a reference for the study of Radix puerariae resource." @default.
- W2378889234 created "2016-06-24" @default.
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- W2378889234 date "2015-01-01" @default.
- W2378889234 modified "2023-09-24" @default.
- W2378889234 title "Simultaneous Determination of Three Isoflavones in Different Kinds of Puerariae Radix by HPLC" @default.
- W2378889234 hasPublicationYear "2015" @default.
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