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- W2378889768 abstract "A two-step PCR screening system of the half-smooth tongue sole Cynoglossus semilaevis BAC library was constructed by an orderly mixing of the BAC clones.Using the specific primers designed to sox9 gene sequence,one positive single BAC clone was screened out from the BAC library.In this study,the sox9-containing BAC clone,P17P21,was mapped to the long arms of a pair of homologous chromosomes of C.semilaevis using the technology of BAC-FISH.The result showed that there are two hybridization signal sites in a pair of autosomes of the female and male C.semilaevi,and the signals are stable and specific.This study confirmed the effectiveness of the two-step PCR screening system and achieved the positioning of sox9 gene on chromosomes of C.semilaevis.The results will facilitate further studies on the sox9 gene of C.semilaevis and will provide an important basis for chromosomal identification of this species." @default.
- W2378889768 created "2016-06-24" @default.
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- W2378889768 date "2014-01-01" @default.
- W2378889768 modified "2023-09-22" @default.
- W2378889768 title "Screening and BAC-FISH locating of the sox9 gene BAC clone of half-smooth tongue sole Cynoglossus semilaevis" @default.
- W2378889768 hasPublicationYear "2014" @default.
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