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- W2378899646 abstract "To isolate Thy-1.1~+ rat bone marrow stem cells by magnetic activated cell sorting(MACS),rat bone marrow cells were collected from rat tibias and femurs,and mononeuclear cells were isolated by Percoll density gradient centrifugation.After incubation with the Thy-(1.1) monoclonal antibody,the Thy-1.1~+ stem cells were purified by indirect magnetic activated cells sorting.The cell purity and viability were evaluated by flowcytometry and typanblue staining respectively and the reclaimation rate was calculated.Simultaneously,the percentage of CD34~+ cells before and after MACS were detected by flowcytometry.Results showed that the purity of Thy-1.1~+ cells was 94.2% after MACS,and the viability was 99.6%,which had no significant difference with the values before MACS(99.8%).The reclaimation rate was(64.7%).The percentage of CD34~+ cells before MACS was 1.2%,with no significant difference from the 1.3% after MACS.High purified Thy-1.1~+ cells were obtained from rat bone marrow using MACS,and the viability of the cells was not impaired.There was no obvious relationship between the Thy-1.1 antigen and the CD34 molecule of rats." @default.
- W2378899646 created "2016-06-24" @default.
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- W2378899646 date "2006-01-01" @default.
- W2378899646 modified "2023-09-24" @default.
- W2378899646 title "Application of Magnetic Activated Cell Sorting in the Isolation of Rat Bone Marrow Stem Cells" @default.
- W2378899646 hasPublicationYear "2006" @default.
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