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- W2379015516 abstract "Objective To clarify the effect of human H1N1 virus on the proliferation and apoptosis in human endothelial cells. Methods After the purified H1N1 virus infected human umbilical vein endothelial cells,virus replication was confirmed by RT-PCR method,the cellular proliferation was determined by MTT,and the apoptosis was detected by flow cytometry analysis and Western blot analysis. Results The number of influenza virus genome in the infected endothelial cells at 6 hours post-infection was increased as compared with no treatment.After being infected with H1N1 virus for 24 hours, the cellular proliferation was significantly inhibited.Many Annein-V staining positive endothelial cells were observed using flow cytometry method.Furthermore,cleaved PRAP was also detected in H1N1 virus-infected cells by Western blot. Conclusions The results show that H1N1 influenza virus can infect and replicate in human umbilical vein endothelial cells and can induce the apoptosis in them." @default.
- W2379015516 created "2016-06-24" @default.
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- W2379015516 date "2011-01-01" @default.
- W2379015516 modified "2023-09-23" @default.
- W2379015516 title "Preliminary Study of H1N1 Influenza Virus Infection on the Apoptosis in Human Endothelial Cells" @default.
- W2379015516 hasPublicationYear "2011" @default.
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