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- W2379021452 abstract "Objective To study the feasibility of chitosanin/plasmid complex-mediated transfection of eNOS in to vascular smooth muscle cells(VSMCs)of wistar rats.Methods PAdTrack-CMV-eNOS harboring green fluorescent protein(GFP)was coated with chitosanin to prepare chitosan/plasmid complexes(CPC);CPC were incubated with cultured VSMCs of Wistar rats aorta under different conditions.The transfection efficiency of CPC was evaluated by detecting GFP and eNOS in the target cells.Transfection with naked PAdTrack-CMV-eNOS plasmid and recombinant Ad-eNOS served as control.Results Evident fluorescence was observed in VSMCs transfected by CPC(highest expression was found at N/P ratio 5:1 at medium pH 7.0)and by Ad-eNOS; Western blotting also showed expression of eNOS protein;no fluorescence was observed in naked PAdTrack-CMV- eNOS transfected group.CPC-eNOS had the highest transfection efficiency of smooth muscle cells when the nitrogen:phosphorus ratio was 5 and the pH value was 7.Conclusion CPC can efficiently mediate gene transfection into VSMCs of Wistar rats and the transfection efficiency is related to the N/P ratio of CPC and the pH value of the medium." @default.
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- W2379021452 date "2007-01-01" @default.
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- W2379021452 title "Chitosanin/plasmid complex-mediated eNOS gene transfer into rat vascular smooth muscle cells in vitro" @default.
- W2379021452 hasPublicationYear "2007" @default.
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