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- W2379040304 abstract "Objective To develop new drugs targeting against peptide deformylase (PDF) of Helicobacter pylori, it is important to prepare a soluble and active PDF in E.coli. Method In this work, PDF gene (def) was cloned into pGEM-T easy vector and transferred to expression vector pET-32a. E. coli cells BL21(DE3) carrying recombinant plasmid of pET-32a-def were induced by IPTG to express PDF. Result BL21(DE3) carrying this plasmid produced high level of fusion protein, with molecular weight of 36kDa determined by SDS-PAGE. Most of the recombinant proteins were expressed as inclusion body, and only small part was soluble. Purified soluble recombinant PDF(rpET-PDF) shew peptide deformylase activity in vitro. Conclusions This work provided the basis for screening of PDF inhibitor." @default.
- W2379040304 created "2016-06-24" @default.
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- W2379040304 date "2005-01-01" @default.
- W2379040304 modified "2023-09-23" @default.
- W2379040304 title "Expression, purification of Helicobacter pylori peptide deformylase in E.coli and its activity determination" @default.
- W2379040304 hasPublicationYear "2005" @default.
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