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- W2379059901 abstract "Objective:To obtain the optimum enzymatic digestion time in Fluorescence in Situ Hybridization.Method:The tissue samples were taken from 42 breast cancerpatients.To establish optimal digestion time, the enzymatic digestion was stopped at 5-minites interval among 12 paraffin-embedded samples.An additional 30 cases were evaluated for FISH with this optimal digestion time.Results:The results showed that the duration of digestion of 10 minutes was not enough and 25 minutes was over digested.Then the optimum time was 15 to 20 minutes, with which the hybridization signals were stronger than the others.Conclusion:We found the optimum enzymatic digestion time in FISH on paraffin-embedded tissues of breast cancerwas 15 to 20 minutes in ourlab.Monitoring digestion time is particularly useful for FISH technique on paraffin-embedded samples." @default.
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- W2379059901 date "2010-01-01" @default.
- W2379059901 modified "2023-09-23" @default.
- W2379059901 title "Research of digestion time in fluorescence in situ hybridization(FISH) technique on paraffin-embedded tissues of breast cancer." @default.
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