Matches in SemOpenAlex for { <https://semopenalex.org/work/W2379071763> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W2379071763 abstract "Objective To construct a recombinant eukaryotic vector bearing the fusion gene of human LIF gene and EGFP(enhanced green fluorescent protein),and to transfect it into human embryonic lung fibroblasts(hELF).Methods The LIF gene was amplified by PCR with the recombinant plasmid pcDNA3.1/ lif as the template and the fragment was combined with plasmid pEGFPN1.The recomninant expression vector,pEGFP-Nl/lif,was transfected to hELF using Lipofectamin.The expressed fusion protein was examined by fluorescence microscopy,RT-PCR and Western-blot.Results The eukaryotic expression vector pEGFP-N1/lif was successfully constructed.The fusion protein EGFP-LIF was detected in hELF.Conclusion The successful construction and expression of the eukaryotic expression vector pEGFP-N1/lif has been achieved." @default.
- W2379071763 created "2016-06-24" @default.
- W2379071763 creator A5086161254 @default.
- W2379071763 date "2007-01-01" @default.
- W2379071763 modified "2023-09-24" @default.
- W2379071763 title "Construction and Expression of Eukaryotic Expression Vector Bearing Fusion Gene of Human LIF Gene and EGFP" @default.
- W2379071763 hasPublicationYear "2007" @default.
- W2379071763 type Work @default.
- W2379071763 sameAs 2379071763 @default.
- W2379071763 citedByCount "0" @default.
- W2379071763 crossrefType "journal-article" @default.
- W2379071763 hasAuthorship W2379071763A5086161254 @default.
- W2379071763 hasConcept C104317684 @default.
- W2379071763 hasConcept C111829193 @default.
- W2379071763 hasConcept C123894998 @default.
- W2379071763 hasConcept C142613039 @default.
- W2379071763 hasConcept C150194340 @default.
- W2379071763 hasConcept C153911025 @default.
- W2379071763 hasConcept C185592680 @default.
- W2379071763 hasConcept C22744801 @default.
- W2379071763 hasConcept C2776415932 @default.
- W2379071763 hasConcept C33161422 @default.
- W2379071763 hasConcept C40767141 @default.
- W2379071763 hasConcept C54009773 @default.
- W2379071763 hasConcept C54355233 @default.
- W2379071763 hasConcept C86803240 @default.
- W2379071763 hasConcept C92087593 @default.
- W2379071763 hasConceptScore W2379071763C104317684 @default.
- W2379071763 hasConceptScore W2379071763C111829193 @default.
- W2379071763 hasConceptScore W2379071763C123894998 @default.
- W2379071763 hasConceptScore W2379071763C142613039 @default.
- W2379071763 hasConceptScore W2379071763C150194340 @default.
- W2379071763 hasConceptScore W2379071763C153911025 @default.
- W2379071763 hasConceptScore W2379071763C185592680 @default.
- W2379071763 hasConceptScore W2379071763C22744801 @default.
- W2379071763 hasConceptScore W2379071763C2776415932 @default.
- W2379071763 hasConceptScore W2379071763C33161422 @default.
- W2379071763 hasConceptScore W2379071763C40767141 @default.
- W2379071763 hasConceptScore W2379071763C54009773 @default.
- W2379071763 hasConceptScore W2379071763C54355233 @default.
- W2379071763 hasConceptScore W2379071763C86803240 @default.
- W2379071763 hasConceptScore W2379071763C92087593 @default.
- W2379071763 hasLocation W23790717631 @default.
- W2379071763 hasOpenAccess W2379071763 @default.
- W2379071763 hasPrimaryLocation W23790717631 @default.
- W2379071763 hasRelatedWork W141783459 @default.
- W2379071763 hasRelatedWork W22278680 @default.
- W2379071763 hasRelatedWork W2350675778 @default.
- W2379071763 hasRelatedWork W2351893465 @default.
- W2379071763 hasRelatedWork W2352482761 @default.
- W2379071763 hasRelatedWork W2353286559 @default.
- W2379071763 hasRelatedWork W2358891865 @default.
- W2379071763 hasRelatedWork W2359188790 @default.
- W2379071763 hasRelatedWork W2364043432 @default.
- W2379071763 hasRelatedWork W2365344696 @default.
- W2379071763 hasRelatedWork W2367055917 @default.
- W2379071763 hasRelatedWork W2374428988 @default.
- W2379071763 hasRelatedWork W2378770117 @default.
- W2379071763 hasRelatedWork W2387805344 @default.
- W2379071763 hasRelatedWork W2393013693 @default.
- W2379071763 hasRelatedWork W3031589081 @default.
- W2379071763 hasRelatedWork W3032304601 @default.
- W2379071763 hasRelatedWork W3142123004 @default.
- W2379071763 hasRelatedWork W650843890 @default.
- W2379071763 hasRelatedWork W929022231 @default.
- W2379071763 isParatext "false" @default.
- W2379071763 isRetracted "false" @default.
- W2379071763 magId "2379071763" @default.
- W2379071763 workType "article" @default.