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- W2379091668 abstract "The aim of this experiment was to select highly competitive and effective rhizobial strains which were compatible with Shengshi alfalfa cultivar by using marker gene technique.The nodules from alfalfa were collected from Yaan in Sichuan,and Beibei,Wanzhou in Chongqing.93 strains were isolated and purified.These strains were then inoculated to on the shengshi of alfalfa by pot experiment in Jensen′s N-free solution.By analysis of the result,4 strains Y6-1-1, BB_1-8-1-1,BB_2-2-1-1 and WZ-6-2-2 were relatively more effective.These strains were selected for further study of competitiveness with soil indigenous rhizobia by using celB marker gene technique.By conjugation with Escherichia coli HAMBI2356 which contains the celB gene,the celB gene were successfully introduced into the four strains respectively.The stability and impact of the insert celB gene on the rhizobial strains were evaluated by determination of generation time,pot inoculation test.The results showed that the marker gene was highly stable,and had no significant effect on the growth,competitiveness and symbiotic effectiveness of the rhizobial strains.So the celB gene marker technique can be used as a perfect means to track the competition of the rhizobial strains in soil.The selected four strains were further assessed for their competitiveness and effectiveness under soil conditions in field experiment.The results showed that three strains Y6-1-1,BB22-1-1,WZ6-2-2 among the four significantly increased the nodule weight,total nodule number,plant shoot dry weight,total nitrogen content,chlorophyll content and yield in comparison with the non-inoculation control.Their nodule occupancy was more than 72%.The best strain was WZ6-2-2,that could be used as inoculant for alfalfa shengshi cultivar." @default.
- W2379091668 created "2016-06-24" @default.
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- W2379091668 date "2006-01-01" @default.
- W2379091668 modified "2023-09-25" @default.
- W2379091668 title "Selection of Highly Effective Rhizobial Strains for Alfalfa" @default.
- W2379091668 hasPublicationYear "2006" @default.
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