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- W2379145386 abstract "To prepare a monoclonal antibody(McAb)against pyruvate dehydrogenase subunit E1β(PDHB)of Mycoplasma gallisepticum,the PDHB was expressed in a prokaryotic system,purified and used as antigen to immunize BALB/c mice.The spleen cells from the immunized mice were fused with mouse myeloma SP2/0cells.Three positive hybridoma cell lines secreting McAbs against PDHB were selected by indirect enzyme-linked immunosorbent assay(ELISA).The antibody titers in the cell culture supernatant and ascites were from 1∶1 024to 1∶2 048and from 1∶12 800to 1∶25 600,respectively.Western-blot and ELISA showed that McAbs reacted with the recombinant PDHB protein and M.gallisepticumtotal protein specifically,and had no cross reactions with other avian pathogens.The immunoglobulin subtypes of McAbs were both IgG1,and light chains wereκ.Three hybridoma cell lines could secret McAbs against PDHB protein stably in vitro.These McAbs were successfully prepared,providing a tool for establishing detection method for M.gallisepticumand further studying biological functions of the PDHB protein." @default.
- W2379145386 created "2016-06-24" @default.
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- W2379145386 date "2014-01-01" @default.
- W2379145386 modified "2023-09-26" @default.
- W2379145386 title "Preparation and identification of monoclonal antibodies against pyruvate dehydrogenase subunit E1β from Mycoplasma gallisepticum" @default.
- W2379145386 hasPublicationYear "2014" @default.
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