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- W2379154569 abstract "In this study,5 strains of Haemophilus parasuis(Hps)were identified by 'satellite' growing test,biochemical and PCR assay.The results showed that aroA gene of the isolated Hps had a high degree of similarity with classical culture strain in the sequence of nucleotide and amino acid sequence,which was between 96.3% to 99.8% and 98.2% to 99.5% respectively.We implemented two program of triplex PCR assay,one group was used to detect simultaneously infections of Hps,PCV2 and PPV,the other group was developed to detect the PCV2,PPV and PRV.The sensitivity tests indicated that the triplex PCR assay may be used for detecting Hps,PPV,PRV and PCV2 of 105 dilute strength;the DNA minimun detectable quantity of Hps,PPV,PRV,PCV2 was 12,16,7.6 and 6.3 pg respectively." @default.
- W2379154569 created "2016-06-24" @default.
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- W2379154569 date "2012-01-01" @default.
- W2379154569 modified "2023-09-23" @default.
- W2379154569 title "Isolation and Identification of Haemophilus parasuis,Cloning and Sequence Analysis of aroA Gene and Institution of Two Program of Triplex PCR Assay" @default.
- W2379154569 hasPublicationYear "2012" @default.
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