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- W2379191167 abstract "The eukaryotic-specific RPL5 is a component of 60S large ribosomal subunit encoded by RPL5 gene.cDNA and genomic sequence of RPL5 were cloned from Giant Panda by RT-PCR;and RPL5 was ectopically expressed in E.coli BL21.RPL5 cDNA cloned from Giant Panda was 918 bp in size,containing 1 open reading frame of 894 bp encoding 295 amino acids.The genomic sequence was found to be 8633 bp long,with eight exons and seven introns.Alignment analysis of nucleotide and deduced amino acid sequences indicated good conservation in Homo sapiens,Bos Taurus,Sus scrofula,Mus altocumulus and Rathauser nonstrategic.Homologies of nucleotide sequence of Giant Panda RPL5 to other species were 94.52%,92.51%,91.95%,91.05% and 89.15%,respectively,while homologies of amino acid sequences were 99.33%,98.65%,98.65%,98.32% and 98.65%,respectively.Molecular weight of the putative RPL5 protein was 34402.6 D with a theoretical PI of 9.73.Topology analysis of Giant Panda RPL5 protein predicted the following functional sites:1 AMP-and GMP-dependent protein kinase phosphorylation site,4 protein kinase C phosphorylation site,2 casein kinase II phosphorylation site,1 tyrosine kinase phosphorylation site and 8 N-mistranslation sites.The giant panda RPL5 gene was expressed in E.coli BL21,with peak expression at 2 h.Fusion protein RPL5-His-tag was about 39 kD." @default.
- W2379191167 created "2016-06-24" @default.
- W2379191167 creator A5075816952 @default.
- W2379191167 date "2013-01-01" @default.
- W2379191167 modified "2023-09-23" @default.
- W2379191167 title "cDNA,GENOMIC SEQUENCE CLONING AND SEQUENCE ANALYSIS OF RIBOSOMAL PROTEIN L5 GENE(RPL5) OF THE GIANT PANDA" @default.
- W2379191167 hasPublicationYear "2013" @default.
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