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- W2379192346 abstract "[Objective] The basis of the development of biological pesticides of anti-virus provided through the research on the inhibition and control effect of the extract from plant on tobacco mosaic virus(TMV).[Method] The 10 times' extract of Oldenlandia was sprayed on tobacco leaf in the 24th hour before and after the leaf of tobacco variety,Xinyeyan and K326 as testing material,was infected by TMV with conventional method of mechanical inoculation.The number of dried spot was counted in the 3rd-5th day after inoculated for the calculation of its inhibition rate.The disease index of ordinary tobacco was investigated after inoculated for the calculation of its control effect.The concentration of virus was measured with the indirect ELISA method.The morphological variation of the virus particle of TMV was observed with negative staining electron microscopy in the different times after the mixture of the purified TMV and the 10 times' extract of Oldenlandia with an equal volume.[Results] The inhibition rate of the extract from Oldenlandia to TMV in Xinyeyan was 84.6% and in K326,76.6%.The inhibition rate of dry spot and anti-host effect were 62.2% and 61.5%,respectively,when the extract was sprayed in the 24th hour before the virus was inoculated.The experiment in the extract's spraying in the 24th hour after virus was inoculated indicated that the proliferation of the virus inside plant could be inhibited.The observation results of electron microscopy showed that the broken phenomenon of virus particles appeared.The longer the mixing time of the purified TMV and the 10 times' extract of Oldenlandia was,the stronger the broken phenomenon of virus particles was.[Conclusion] The Oldenlandia also had a strong inhibitory effect on TMV,and the longer the acting time was,the higher the inhibition rate was." @default.
- W2379192346 created "2016-06-24" @default.
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- W2379192346 date "2010-01-01" @default.
- W2379192346 modified "2023-09-27" @default.
- W2379192346 title "Research on the Resistant Mechanism of the Herba hedyotis diffusae Willd to Tobacco Mosaic Virus" @default.
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