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- W2379211550 abstract "According to silkworm EST generated by the large-scale cDNA sequencing in our lab,we found an EST which is highly homologous to the eIF3f.By using the methods of electronic clone and 3′RACE,a full-length cDNA,named as eIF3f(GenBank accession number DQ868530) was isolated from silkworm,which is 1 009 bp nucleotide long,and contains an ORF(870 bp) encoded 289 amino acids,55 bp nucleotide sequence in 5′-UTR and 65 bp nucleotide sequence in 3′-UTR.The deduced amino acids showed a high degree of similarity to the previously reported eIF3fs and shared a conserved JAB-MPN domain which is essential for the translation initiation.Genomic structure analysis suggested that eIF3f contains 5 exons and 4 introns.In the 5′upstream,many potential binding sites for the transcription factors were detected,such as E74A,DFD, DRI etc,but no TATA box.The expression level of eIF3f gene at different stages and in different tissues were different.The results implied that eIF3f is a negative regulator of translation initiation.The above results will be helpful to elucidate the regulation principle of expression of genes related to eukaryotic translation iniuiation factors." @default.
- W2379211550 created "2016-06-24" @default.
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- W2379211550 date "2008-01-01" @default.
- W2379211550 modified "2023-09-25" @default.
- W2379211550 title "Analysis of Structure and Expression of Eukaryotic Translation Initiation Factor 3f Gene in the Silkworm,Bombyx mori" @default.
- W2379211550 hasPublicationYear "2008" @default.
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