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- W2379277795 abstract "In order to study the effect of over-expression of hDaxx on the dexamethasone-induced apoptosis in macrophages, macrophages were transfected with eukaryotic expression vectors pEGFP/hDaxx and pEGFP respecrtively, and the expression of Daxx was identified by Western blotting. In addition, the expression and localization of GFP-hDaxx fusion protein in macrophages were observed under fluorescent microscopy. Then, dexamethasone was added to macrophages to induce apoptosis, and the changes on cellular morphology were observed by Giemsa staining, and the apoptotic rate of macrophages was there by calculated. It was found that GFP-hDaxx fusion protein was successfully expressed on macrophages and localized in nuclei of macrophages. The presence of GFP-hDaxx fusion protein did not interfere with the specificity of Daxx. Besides these, there was a remarkable difference between pEGFP/hDaxx group and the dexamethasone control group and pEGFP control group, all of these indicated that transient over-expression of hDaxx could decrease the apoptotic rate of macrophages induced by dexamethasone." @default.
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- W2379277795 date "2004-01-01" @default.
- W2379277795 modified "2023-09-27" @default.
- W2379277795 title "Effect of Over-Expression of hDaxx on the Dexamethasone-Induced Apoptosis in Macrophages" @default.
- W2379277795 hasPublicationYear "2004" @default.
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