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- W2379331728 abstract "Objective To investigate the growth characteristics of PC12 cells and detect the level of secretion and the expression of the proenkephalin mRNA in serial subcultivation, and to provide the evidence of cell transplantation in pain treatment. Methods PC12 cells were grown in DMEM supplemented with 15% fetal bovine serum. The norepinephrine(NE) in the medium was measured with high-performance liquid chromatography -electrochemical detection technique (HPLC-ED), and methionine-enkephalin(M-EK) was measured with Radioimmunoassay. The proenkephalin(PENK) mRNA expression in PC12 cells were detected by reverse transcription-PCR. Results The levels of basic secretion of NE and M-EK in the medium were (465.8±78.5)pg/mL and (4.3±0.66)pg/mL respectively. The PENK mRNA in PC12 cells were low expression. Conclusion The PC12 cells cultured in vitro could synthesis and secrete NE and M-EK, and the expression of PENK mRNA was positive. It will provide a new cell resource of cell transplantation in pain treatment." @default.
- W2379331728 created "2016-06-24" @default.
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- W2379331728 date "2008-01-01" @default.
- W2379331728 modified "2023-09-24" @default.
- W2379331728 title "Characteristics of secretion and gene expression of PENK in serial subcultivated PC12 cells" @default.
- W2379331728 hasPublicationYear "2008" @default.
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