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- W2379411489 abstract "Objective To establish an analytical method for the determination the concentration of linezolid in vitro pharmacodynamic model. Methods Linezolid was precipitated from MH broth with hydrochloric acid. HPLC-UV method was carried on with a venusil XBP C8 (4.6 mm×250 mm, 5 μm) column. The mobile phase consisted of water-acetonitrile (80∶20). The flow rate was 1.5 mL·min-1 and the detection was performed at 251 nm. Results The calibration curve was linear with in the range of 0.5-40.0 μg·mL-1 (γ=0.9996). The absolute recoveries of linezolid at the concentrations of 2,10, 40 μg·mL-1 were all higher than 90% , and the relative recoveries were from 96%to 98%. The RSDs of the within-day and between-day variations were lower than 2% and 4% , respectively. Conclusion This HPLC-UV method was simple, sensitive and accurate, and suitable for routine determination the concentration of linezolid for vitro pharmacodynamic study." @default.
- W2379411489 created "2016-06-24" @default.
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- W2379411489 date "2010-01-01" @default.
- W2379411489 modified "2023-09-27" @default.
- W2379411489 title "Determination the concentration of linezolid in Mueller hinto broth in vitro pharmacodynamic model by HPLC-UV" @default.
- W2379411489 hasPublicationYear "2010" @default.
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