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- W2379463452 abstract "[Objective] The aim was to lay the foundation for further study on the related fields such as integration efficiency of transgenic pig production,expressive level of foreign target gene,specific expressive distribution of target gene in the tissue of pig and so on.[Method] The transgenic pig expression vector PM1 marked with GFP gene was introduced into the 1-cell or 2-cell embryos of Hubei White pigs and Tonghu pigs by microinjection to produce piglets by embryonic implantation.The total DNA from genome was extracted from the pig ears and tails and the integration situation of PM1 in the pig genome was detected by PCR. [Result] Among the 78 produced piglets,there were 6 transgenic positive pigs detected by PCR and the amplified target fragment of 650 bp from the 6 pigs was all obtained by 3 PCR amplified reactions.Other samples which did not obtain the amplified target fragment were negative pigs.[Conclusion] The PM1 vector could be used to carry foreign genes to produce the transgenic pigs." @default.
- W2379463452 created "2016-06-24" @default.
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- W2379463452 date "2008-01-01" @default.
- W2379463452 modified "2023-09-23" @default.
- W2379463452 title "Study on Integration Detection of Transgenic Pig Marked with Green Fluorescent Protein" @default.
- W2379463452 hasPublicationYear "2008" @default.
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