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- W2379593485 abstract "Objective To design and synthesize the cDNA fragment of hammerhead ribozyme, which was hepatitis C virus core gene RNA-specific, and investigate its extracellular cleavage of hepatitis C virus core gene RNA. Methods Design the ribozyme cDNA fragment and construct recombinants. Clone HCV core gene cDNA to pGEM-7Zf (+) plasmids. Get ribozyme RNA and HCV RNA through in vitro transcription. Mix ribozyme RNA, HCV RNA, ribozyme reaction buffer and RNasin in certain ratio. Incubate them at 37℃. Analyze the result by polyacrylamide gel electrophoresis and auto photographing. Result Both white and blue bacteria groups could be seen in the white and blue scanning system. Sequencing showed the sequence from 130 to 82 was combinative to ribozyme cDNA. As to HCV core gene recombinant, after EcoRⅠ and Hind Ⅲ cleavage, a band could be seen at about 410bp on agarose gel electrophoresis. Auto-photographing showed HCV RNA had only one band at 453nt. After ribozyme reaction, however, three bands could be seen when the incubation time was 15 minutes or 30 minutes, which were 453nt, 307nt, 146nt, while only two bands were seen at 307nt and 146nt when the incubation time was 60 minutes. Conclusion The result showed that the HCV core gene RNA-specific hammerhead ribozyme expression vector had been successfully constructed, and the ribozyme could cleave HCV RNA." @default.
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- W2379593485 date "2001-01-01" @default.
- W2379593485 modified "2023-09-23" @default.
- W2379593485 title "Extracellular cleavage of viral RNA by hepatitis C virus core gene RNA specific hammerhead ribozyme" @default.
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