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- W2379602093 abstract "[Objective]The purpose of this project aimed to provide technological support for the Seaisland cotton transgenic breeding by exploring a more efficient and convenient genetic transformation technology.[Method]Four Sea-island cotton varieties were used for genetic transformation with the method of agrobacterium infecting flower stigmas at different points of infection time.Then their cotton boll rate,field kanamycin resistance rate were counted,and the positive strains rate were analyzed by PCR.[Result]The corresponding average rate were 12.60%,9.87%and 1.72%.Of four points of the infection time,12 hours after anthesis showed that the highest cotton boll rate,field kanamycin resistance rate and positive strain rate were 20.25%,11.68%and 2.59%respectively.[Conclusion]The results showed that the best time of flower stigmata being infected with agrobacteria for Sea - island cotton genetic transformation was 12 hours after anthesis." @default.
- W2379602093 created "2016-06-24" @default.
- W2379602093 creator A5076414203 @default.
- W2379602093 date "2013-01-01" @default.
- W2379602093 modified "2023-09-25" @default.
- W2379602093 title "Agrobacterium Infecting Flower Stigma for Sea-island Cotton(Gossypium barbademe L.) Genetic Transformation" @default.
- W2379602093 hasPublicationYear "2013" @default.
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